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Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria
Supernatant, which served as an extraction and inhibition control agent, before nucleic acid was extracted using the CAS1820 XtractorGene automated system (Qiagen-Australia) according to the manufacturer's instructions. The final volumes of specimen extracts were 150 L/specimen eluted in 96 well racks (Matrix, Thermo Scientific, Australia). For each run (96 extracts/run), extracts were tested usi


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Er or others), season specimen collected, and time from specimen collection to being frozen in the laboratory. Analyses were conducted using Stata statistical software v.11.0 (StataCorp, College Station, TX, USA).however 10.9 of swabs were received more than 7-days after their collection.Excluded samples:For EHV1 extraction and inhibition testing, 42 (0.81 ) DNA extracts failed the EHV1 criteria
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