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Leaved form of PARP (Figure 1E). Taken together, these results indicate that apicularen A induces apoptotic cell death in HeLa cells.PMA increases apicularen A-induced cytotoxicity in HeLa cellsResults are expressed as the means ?SE. Statistical significance was assessed using the Student's t-test and analysis of variance (ANOVA). P
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D 10 l of a 50 slurry of Ni2 -NTA-agarose were then added, and the mixture was rotated at 4 for 1 h. Agarose beads were washed with Buffer F (50 mM Tris-HCl (pH 7.5), 300 mM NaCl, 0.2 Tween 20) five times, and DnaK-His and 32 were dissociated from the agarose beads by treating the precipitate with 250 mM imidazole. Proteins were subjected to SDS-PAGE and detected by CBB staining. Experiments
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T was completely absorbed at 120 DPI. The collagen fibers have high density with an aligned direction in the line of stress between the muscle and calcaneus. No obvious degeneration is seen and the cellular and collagenic structures are highly matured (B) similar to the intact tendons (C). The gastrocnemius muscle in the injured control legs shows muscle atrophy with the newly regenerated granula
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